Figure 6

ARC expression in clinical samples in patients with CRC. (a) Box plot showing correlation between ARC expression and disease stage in patients with CRC in TCGA dataset. Stage 0–II: n = 271; stage III: n = 133; stage IV: n = 62. Within all samples, we analyzed samples which had complete TNM clinical stage information. Asterisks denote significant difference (*p < 0.05). (b) Kaplan–Meier curves for overall survival in patients with CRC according to expression of ARC (p = 0.013). Patients are divided into two groups by the median. These data were obtained from Broad GDAC Firehose colorectal adenocarcinoma (COADREAD) dataset (https://gdac.broadinstitute.org/) (n = 615). (c) IHC of ARC in tumor and normal tissue in a specimen of CRC. Scale bars indicate 1 mm (left) and 200 µm (right). (d) IHC of ARC in the deepest parts of tumor in a specimen of CRC. Scale bars indicate 1 mm (left) and 200 µm (right). (e) Kaplan–Meier curves for relapse-free survival in patients with CRC according to ARC expression. Patients are divided into three groups by the intensity of ARC expression in IHC (n = 100). CRC colorectal cancer, TCGA The Cancer Genome Atlas, IHC immunohistochemical staining.