Figure 5
Effect of DC-SIGN pre-exposure on anti-IgM-induced endocytosis. (a) WSU-FSCCL cells incubated with AF488-conjugated anti-IgM (5 μg/ml) at 4 or 37 °C or were left untreated for 30 min. Then cells were incubated with anti-AF488 antibody (30 min at 4 °C to quench AF488 remaining on the surface), or left untreated. Graph on the left shows cells with no pre-treatment, whereas graphs in the middle and right show results for cells that were pre-treated for 30 min at 4 °C with DC-SIGN-Fc or DC-SIGN-HA respectively, prior to anti-IgM stimulation.
