Figure 3 | Scientific Reports

Figure 3

From: Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

Figure 3

Nobiletin effect in blastocysts lipid content. (a) Quantification of the total area of lipid droplets (µm2) in Day 7 blastocysts cultured in SOF + 5% FCS (Control), supplemented or not with 5 (Nob5) or 10 µM (Nob10) nobiletin or with 0.03% dimethyl sulfoxide (CDMSO) during 21–54 hpi (MNEGA: presumptive zygote to 8-cell stage) or during 54–96 hpi (MJEGA: 8- to 16-cell stage). Data are the mean ± s.e.m. Significant differences (P < 0.001) are indicated with different letters. (b) Representative fluorescence images of lipid droplets in Day 7 blastocysts from all experimental groups (Control, Nob5 Nob10, CDMSO) in both phases (MNEGA or MJEGA). Images were captured on 63× objective. Scale bar 50 µm.

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