Figure 2 | Scientific Reports

Figure 2

From: A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities

Figure 2

Independent analysis of glycoside hydrolase and lignin modifying enzyme activities Conversion of (A) GH probe (n = 3) and (C) LME probe (n = 3). For both assays, enzyme loading concentrations were 0.74 μg and 0.15 μg per 0.83 mM probe for Mt and Ab, respectively. The conversion ratio is expressed as the relative intensity of product and substrate peaks. (B) Conversion of oxime-NIMS-tagged oligosaccharides (n = 3). Enzyme loading concentrations were 1.4 μg and 0.3 μg per 1 mM xylotetraose or cellotetraose for Mt and Ab, respectively. The conversion ratio is expressed as the mass ratio of products, determined by normalization to C13 internal standards.

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