Figure 3

Multiplex assays against mixed GH and LME probes (n = 3). (A) Conversion of GH probe in the presence of LME probe. (B) Conversion of LME probe in the presence of GH probe. Enzyme loading concentrations were 0.74 μg and 0.15 μg per 1.66 mM total substrate (0.83 mM GH probe and 0.83 mM LME probe) for Mt and Ab, respectively.