Figure 4 | Scientific Reports

Figure 4

From: A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities

Figure 4

Effect of mediators and inhibitory conditions on cellotetraose degradation by (A) Mt (B) Ab and (C) CelE CBM3a (n = 3). “*” indicate ANOVA post hoc significant differences compared to enzyme only. Enzyme loading concentrations were 1.4 μg and 0.3 μg per 1 mM cellotetraose for Mt and Ab, respectively. (G1 = glucose; G2 = cellobiose; G3 = cellotriose; G4 = cellotetraose) Inhibition reaction was run at 37 °C for 20 min; Oxime tagging reaction time = 22 h.

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