Table 1 Primers used in this study.
Experiments | Primer name | Primer sequences(5′–3′) | Primer size (bp) | Tm (℃) | GC% | Amplicon size (bp) |
|---|---|---|---|---|---|---|
Full length PCR amplification | TpHSP70-1F | CAAGCTACCAAACGCGACTAACC | 23 | 55 | 52 | 766 |
TpHSP70-1R | AAAGGAGCCGCAATGTTGAATG | 22 | 57 | 45.5 | ||
TpHSP70-2F | AAAGGAAGAGGTTGGCAAGGAA | 22 | 56 | 45.5 | 768 | |
TpHSP70-2R | CAGTTGAAGGAAGGGTAGAGTGGA | 24 | 55 | 50 | ||
TpHSP90F | TACTCTACGCTTTACATCCGAACAC | 25 | 49 | 44 | 750 | |
TpHSP90R | GAACAACTTAATCAACCTCCTCCAT | 25 | 49.5 | 40 | ||
Hsp70-3'-RACE | 3TpHSP70-1F1 | CACCAAGATGAAGGAGACCGCCGAG | 25 | 60 | 60 | 1741 |
3TpHSP70-1F2 | CAAGAAGACCCAGGGCGAGAAGAAC | 25 | 59 | 56 | ||
3TpHSP70-2F1 | ACAAGAAGGAGGGCGAGAAGAAC | 23 | 57 | 52.2 | 1140 | |
3TpHSP70-2F2 | GATCGACAATGAAGCCCGTGCAG | 23 | 59 | 56.5 | ||
Hsp70-5'-RACE | 5TpHSP70-1R1 | GATCGACTTGTTGAGCTCCTTGCC | 24 | 58 | 54.2 | 1095 |
5TpHSP70-1R2 | GGCACGGGTGATCGAGGAGTAGAA | 24 | 60 | 58.3 | ||
5TpHSP70-2R1 | GCTGCGAATGTCCTTGCCGGTCT | 23 | 61 | 60.9 | 890 | |
5TpHSP70-2R2 | CCACCGCCCAGATCGAACACCAG | 23 | 63 | 65.2 | ||
Hsp90-3'-RACE | 3TpHSP90F1 | CGTCAAGAAGTGCCTGGAGCTGT | 23 | 58.5 | 56.5 | 747 |
3TpHSP90F2 | TGAGGAGAAGAAGAAGCGCGAGG | 23 | 58.5 | 56.5 | ||
Hsp90-5'-RACE | 5TpHSP90R1 | CATGTAGACGACCTCGAAGCCAC | 23 | 58.5 | 56.5 | 1402 |
5TpHSP90R2 | CGTCAAACAGCTCCAGGCACTTC | 23 | 58.5 | 56.5 | ||
Real-time PCR | qTpHSP90F | TTCCAGTGGCGACGAGATGT | 20 | 60.8 | 55 | 180 |
qTpHSP90R | TCCTCAGGCAGCTCCAGACC | 20 | 62 | 65 | ||
qTpHSP70-1F | CACTAACGACAGGCGATTCTACG | 23 | 59.4 | 52.2 | 150 | |
qTpHSP70-1R | TCAACCGAACAACCACTCAAGC | 22 | 58.5 | 50 | ||
qTpHSP70-2F | GAACTGGTCCTGCTCGATGTGAAC | 24 | 60.5 | 54.2 | 120 | |
qTpHSP70-2R | ACCCTCGAAGACTTGAATTGTGACG | 25 | 57 | 48 | ||
qTpTubulinF | TATCCTCGCATTCACTTTCCTCTGG | 25 | 57 | 48 | 100 | |
qTpTubulinR | CGAAGCAGGTGTTGGTAATCTCAGC | 25 | 59 | 52 |
