Table 1 Combinations of Cytokines used for expansion of fetal liver hematopoietic stem and progenitor cells.
From: In vitro expansion of fetal liver hematopoietic stem cells
Cytokine used in Combinations | Combination 1 (M) | Combination 2 (M + S) | Combination 3 (M + S + M1) | Combination 4 (M + S + M2) | Combination 5 (M + S + M3) | Combination 6 (M + S + M4) |
|---|---|---|---|---|---|---|
Media | Media | Media | Media | Media | Media | Media |
Supplements | Supplements | Supplements | Supplements | Supplements | Supplements | |
SCF | 100 ng/ml | 100 ng/ml | 100 ng/ml | 100 ng/ml | ||
FLT-3 | 100 ng/ml | 100 ng/ml | 100 ng/ml | 100 ng/ml | ||
IL-6 | 20 ng/ml | 20 ng/ml | 20 ng/ml | |||
EPO | 2U/ml | 2U/ml | ||||
GM-CSF | 200U/ml |
