Figure 4
From: High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay
Quantitation. (A) Two positive control IgG mAbs and a negative control mAb were titrated to determine the linear range of the mix-and-read signal. (B) Five serum samples that gave a strong signal in initial testing (503–1179 fold over background) were titrated in negative human serum to determine if they reached the saturation level identified by mAbs. (C) Correlation of ELISA end-point titer and ELISA optical density from 1:100 sample dilution. (D) Correlation of ELISA end-point titer and mix-and-read signal. Correlation analysis (Pearson r and 95% confidence interval) and linear regression were performed on log-transformed mix-and-read data and log2-transformed ELISA end-point titers. (A) and (B): technical duplicates with averages and ranges presented.
