Figure 3 | Scientific Reports

Figure 3

From: Rare CASP6N73T variant associated with hippocampal volume exhibits decreased proteolytic activity, synaptic transmission defect, and neurodegeneration

Figure 3

Casp6WT and Casp6N73T enzymatic processing of Ac-VEID-AFC. (a) VEIDase activity of Casp6WT or Casp6N73T at 2, 10, 20, 50, 100, or 400 nM on 20 µM Ac-VEID-AFC. (b) Cleaved Ac-VEID-AFC generated with time by Casp6WT or Casp6N73T at 2, 10, or 50 nM on 20 µM Ac-VEID-AFC. Data represents mean and s.e.m. from 3 independent experiments. No statistical differences were found by two-way ANOVA (a) or repeated measures two-way ANOVA (b). (c) VMAX, Km, kcat, and kcat/Km values for Casp6WT and Casp6N73T. Data represents mean and SD from 3 independent experiments.

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