Figure 1
The bacterial fermentation with polymer was accompanied by the decrease in intracellular pH. (A)The color change of phenol red from red to yellow when C. acnes was incubated with/without PF68 in TSB. (B) The prevalence of fermentation was quantified by measuring the optical density of phenol red at OD560. The pH of media with PF68 and C. acnes was significantly lower than the other groups. (C) The ion chromatogram and mass spectrum showing the SCFAs present in the C. acnes ferment cultured with PF68. (D) Quantification of SCFAs in the C. acnes ferment filtrate. A high amount of PA was produced by PF68 fermentation of C. acnes. Medium (M), C. acnes (B), acetic acid (AA), propionic acid (PA), butyric acid (BA), and iso-butyric acid (I-BA). Data are the mean ± SD from three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ns = non-significant (two-tailed t-test).
