Figure 2 | Scientific Reports

Figure 2

From: Propionic acid produced by Cutibacterium acnes fermentation ameliorates ultraviolet B-induced melanin synthesis

Figure 2

The effects of tyrosinase gene expression and cell proliferation in melanoma cells after PA treatment. (A) Tyrosinase gene expression decreased in PA treated B16F10 melanoma cells quantified by RT-qPCR. (B) The effects of PA on B16F10 melanoma cells evaluated by BrdU labeling. PA treatment did not alter the cell proliferation. (C) Effects of PA and AA in melanocytes. The relatively higher efficacy of PA in attenuating melanin content. (D) Blocking the signaling of FFar2 using GLPG prevents the PA mediated decreased cellular tyrosinase activity in B16F10 cells. Control (C), propionic acid (PA), acetic acid (AA), dimethyl sulfoxide (DMSO), and GLPG0974 (GLPG). Data are the mean ± SD from three separate experiments. *P < 0.05, **P < 0.01 and ns = non-significant (two-tailed t-test). Scale bar = 120 µm.

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