Figure 3
Induction of melanocytes by UVB and inhibition by different treatments. (A) The ears of mice were applied PF68 + C. acnes. The same amount of PBS, PF68 only, and C. acnes only were applied as control. Specimens were examined microscopically for the effects of PF68 + C. acnes treatment on the DOPA-positive melanocytes proliferation in the epidermis. (B) The ears of mice were applied PA. The same amount of PBS was applied as control. Specimens were examined microscopically for the effects of PA treatment on the DOPA-positive melanocytes proliferation in the epidermis. (C) Tyrosinase gene expression after PA treatment in vivo was quantified by RT-qPCR. Control (C), C. acnes (B), propionic acid (PA). The arrowhead in A and B shows the staining of DOPA-positive melanocytes that was counted in the same millimeter squared. Data are the mean ± SD from three separate experiments was replayed. *P < 0.05, ***P < 0.001 (two-tailed t-test). Scale bar = 60 μm.
