Figure 4
The siRNA knockdown efficiency of the FFAR2 gene, along with the topical application of PA and UV exposure to ICR mice ears. (A) Specimens were examined microscopically for the effects of PA treatment after FFAR2 gene knockdown on the DOPA-positive melanocytes proliferation in the epidermis. (B) Tyrosinase gene expression in vivo quantified by RT-qPCR. (C) Western blotting to confirm FFAR2 gene knockdown and the expression of FFAR2 and β-actin in mice ears. Full-length gels images are available in Supplemental Information. Control (C), propionic acid (PA), negative control (NC). The arrowhead in A and B shows the staining of DOPA-positive melanocytes that was counted in the same millimeter squared. Data A and B are the mean ± SD from three separate experiments except two independent western blotting analysis for C was replayed. *P < 0.05, ***P < 0.001 (two-tailed t-test). Scale bar = 60 μm.
