Figure 4 | Scientific Reports

Figure 4

From: Tissue microarray profiling and integrative proteomics indicate the modulatory potential of Maytenus royleanus in inhibition of overexpressed TPD52 in prostate cancers

Figure 4

Expression of TPD52 in prostate cancer. (a) Expression of TPD52 in prostate cancer progression model. RWPE < WPE2-NA22 < WPE1-NB14 < WPE1-NB11 < WPE1-NB26 with increase in proliferation and progression of cancer protein expression of TPD52 also increased as depicted by Immunoblot analysis. The total cell lysate was prepared and 40 μg protein was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. (b) Expression of TPD52 was observed in seven prostate cancer cell lines (RWPE, LnCap, Du145, CWR22Rν1, PC3, C4-2, and NB26) under regular culture conditions. Except for RWPE-1 all other cell lines tested showed marked protein expression of TPD52 in Immunoblot analysis. (c) Immunoblot analysis for expression of TPD52 in prostate cancer development and progression model (transgenic adenocarcinoma of the mouse prostate, TRAMP), showed an increase in expression with the development and progression of prostate cancer. The blots shown here are representative of three independent experiments with similar results.

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