Figure 5
Validation of TPD52 expression in C4-2 and CWR22Rν1 cells and effect of MEM treatment on TPD52 expression in both cell lines. (a) Immunoblot analysis of TPD52 expression of C4-2 and CWR22Rν1 in MEM treated group as compared to the control group. The total cell lysate was prepared and 40 μg protein was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin, the experiment was done in triplicate. (b) Modulation of TPD52 expression by MEM treatment in CWR22Rν1 (b1) and C4-2 (b2) cells. mRNA expression of TPD52 in MEM treated CWR22Rν1 and C4-2 cells (RT-PCR), experiment performed in triplicate (mean ± SD), *, p < 0.01, **, p < 0.05. (c) Immunofluorescence staining of C4-2 and CWR22Rν1 cells demonstrating expression of TPD52 in both MEM treated (40 mol/L) of C4-2 (c2) and CWR22Rν1 (c4) cells as compared to control C4-2 (c1) and CWR22Rν1 (c3) cells (untreated). Alexa fluor staining of TPD52 of both cell lines (green fluorescence) and counterstained with DAPI (blue fluorescence) were observed.
