Figure 7 | Scientific Reports

Figure 7

From: Tissue microarray profiling and integrative proteomics indicate the modulatory potential of Maytenus royleanus in inhibition of overexpressed TPD52 in prostate cancers

Figure 7

Validation of TPD52 expression in CWR22Rν1 xenograft tissues in athymic nude mice and the effect of MEM treatment on TPD52 expression. (a) Immunoblot analysis of TPD52 expression CWR22Rν1 xenograft tissues in MEM treated group as compared to the control group. The total cell lysate was prepared and 40 μg protein was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. The blots shown here are representative of three independent experiments with similar results. (b) Immunohistochemistry of CWR22Rν1 xenograft tissues demonstrating expression of TPD52 in control (untreated b.1) and MEM (2.5 mg) treated mice (b.2). An intense brown stain of the cytoplasm of control tissues showed high expression of TPD52 as it is cytoplasmic in origin as compared to MEM treated mice tissue where there is no staining observed.

Back to article page