Figure 5

Synaptic AMPAR functions are impaired in mPFC TARP γ-8 knockout mice. (a–d) mEPSCs from γ-8^+/− and γ-8^−/− mPFC pyramidal cells had reduced amplitude but unchanged frequency. (a) Cumulative frequency distribution of mEPSC amplitudes. Insert shows sample traces. Scale bar, 5 pA, 5 ms. (b) Amplitude of mEPSC was reduced in mPFC pyramidal cells from γ-8^+/− and γ-8^−/− mice. (c) Cumulative frequency distribution of mEPSC interevent interval. Sample traces are shown as insert. Scale bar, 10 pA, 500 ms. (d) Frequency of mEPSC did not differ among WT, γ-8^+/− and γ-8^−/− neurons. (n = 12 for WT; n = 13 for γ-8^+/−; n = 13 for γ-8^−/−). (e) AMPA/NMDA ratio was reduced in mPFC pyramidal neurons from γ-8^+/− and γ-8^−/− mice (n = 14). Representative EPSCs recorded at holding of − 70 mV and + 40 mV are shown above the bar graph. Arrows indicate where NMDAR-EPSCs were measured. Scale bar, 50 ms. (f) Paired-pulse ratio (PPR) does not differ between WT, TARP γ-8^+/− and TARP γ-8^−/− neurons (n = 8 for each). The sample traces are shown above the bar graph. Scale bar, 50 pA, 100 ms. Data represent the mean ± s.e.m. one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001.