Figure 1 | Scientific Reports

Figure 1

From: ALS-linked FUS mutants affect the localization of U7 snRNP and replication-dependent histone gene expression in human cells

Figure 1

Localization of FUS and U7 snRNA in cells transfected with plasmids carrying FUS WT and ALS-linked FUS mutations. Fluorescent in-situ hybridization (FISH) using a probe against U7 snRNA in combination with immunofluorescence (IF) using anti-FLAG antibodies was performed in SH-SY5Y FUS KO (A,B) and HeLa FUS KO (C) cells transfected with FLAG-tagged FUS. DAPI was used for nuclear staining. Graphs show the percentage of transfected cells with mislocalization of FUS and U7 snRNA. WT FUS—cells transfected with plasmids encoding the wild-type FUS gene. P525L and R495X—cells transfected with plasmids encoding the FUS gene with P525L and R495X mutations.

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