Table 1 Performance of primers of the intercalating dye-based RT-qPCR protocol for SARS-CoV-2 detection.
From: Complementary methods for SARS-CoV-2 diagnosis in times of material shortage
Gene | Primer concentration | 58 °Ca | 60 °Ca | ||
|---|---|---|---|---|---|
Tm (oC) | LOD (copies) | Tm (oC) | LOD (copies) | ||
E | Fwd, Rv: 200 nM | – | – | 81.02 (± 0.07) | 50 |
Fwd, Rv: 400 nM | 81.06 (± 0.03) | 50 | 81.07 (± 0.02) | 5 | |
RdRp | Fwd: 600 nM Rv: 800 nM | 80.6 (± 0.03) | 1000 | 80.6 (± 0.06) | 100 |
