Figure 2 | Scientific Reports

Figure 2

From: CLDN15 is a novel diagnostic marker for malignant pleural mesothelioma

Figure 2

Establishment of a novel anti-CLDN15 mAb suitable for IHCs. a Schematic representation of the domain structure of human CLDN15 protein. The region corresponding to the peptide used for the immunization is shown in orange. b Amino-acid sequence of the cytoplasmic tail of CLDN15 and alanine mutants used in this study. Alignment with other CLDNs is also shown. c IHC of HEK293T cells expressing CLDNs. Note that 2C11 specifically stains only CLDN15-expressing cells. d Epitope analysis of 2C11. Note that 2C11 signal is lost in mutants 9–19, indicating that the epitope is 219FGKYGRNA226 of CLDN15. e Amino-acid sequences of CDRs of 2C11.

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