Figure 5
(a) p-ERK1/2 protein expression is shown by representative western blots and scatter plots. The protein expression of p-ERK1/2 was normalized to β-actin (loading control). In all comparisons, the average value for the control group was set to 100%. p-ERK1/2 has a molecular weight of 42/44 kDa and the one of β-actin is 43 kDa. Data are presented as median ± IQR, *P < 0.05 is considered statistically significant. (b) Representative image of pERK1/2 immunoreactivity. Upper panel, saline treated animal. To the left, area of stroke (outlined in red) is illustrated in a large image. Letters a1 and b2 refer to the illustrations to the right. a1. The stroke core is shown. Arrows point at pERK1/2 immunoreactive cells and arrow heads at vessels. b2. Immunoreactive cells and vessels were also found close to the stroke area. Lower panel, t-PA + U0126 treated animal. To the left, red-outlined stroke areas is illustrated in a large image. Letters a1 and b2 refer to the illustrations to the right. a1. Some pERK1/2 immunoreactive cells (arrows) were found in the stroke core, but not in the area outside the stroke b2. (c) Scatter plot showing semi-quantification of protein expression for pERK1/2. Data are presented as median ± IQR, and normalized to the non-occluded side. *P < 0.05.
