Figure 3 | Scientific Reports

Figure 3

From: Loss of the transcriptional repressor Rev-erbα upregulates metabolism and proliferation in cultured mouse embryonic fibroblasts

Figure 3

Rev-erbα stabilization reduces levels of glycolysis as well as glycolytic and PPP enzyme expression. (A) Western Blot analysis to measure Rev-erbα protein levels in Rev-erbα KO, SD, and WT cells. β-Actin was used as a loading control. Densitometry analysis of Rev-erbα levels in Rev-erbα KO, SD, and WT cells. n.d. denotes not detected. (B) Proton efflux rate trace for WT and Rev-erbα SD MEFs normalized to cell number. Anti/Rot: antimycin A/rotenone. Levels of basal and compensatory glycolysis represented as ratios to WT. (C) Fuel dependency analysis in WT and Rev-erbα KO MEFs. Glutamine, fatty acid, and glucose dependency are denoted as a percentage of total fuel oxidation. (D) Expression of TKT, RPIA, and pdhb1 genes in WT and Rev-erbα SD cells. n = 3 independent experiments. Error bars represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus WT.

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