Table 2 Sequence alignment of the novel, not described previously (vian 1–20) and known (varv A, cyO2, viba 12) cyclotides found in V. anagae.

From: Cyclotide host-defense tailored for species and environments in violets from the Canary Islands

  1. Novelty or identity of the sequences has been established by comparing them to sequences deposited in Cybase (http://www.cybase.org.au) and Uniprot (https://www.uniprot.org/).
  2. aSequenced by MSMS on the peptide level. X in the sequence stands for either I or L.
  3. bMasses not found in mass spectra—undetermined N-terminal cleavage position from the precursor peptide.
  4. cThe peptide tail sequence recognized by the cyclization enzyme is present in the precursor protein.
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