Figure 5

Effect of YIV-906 on the action of INFg or IL4 on polarizing bone marrow derived macrophage (BMDMs) into M1 or M2-like macrophage. (A) Heat map for the mRNA expression levels of BMDM following INFg or IL14 with or without YIV-906/GU treatment. For each row (gene), up-regulation of mRNA is highlighted as (red) while down-regulation is highlighted as (green). Number of the table shows the relative fold change gene expression in each treatment conditions (average of three independent experiment and all gene expressions were normalized to actin). (B) Western blot analysis for the effect of YIV-906GU on the action of IFNg on IFNg signaling of BMDMs. Cropped blots are used in this figure and they have been run under the same experimental conditions. (C) Western blot analysis for the effect of YIV-906GU on the action of IL4 on IL4 signaling of BMDMs. Bone marrow cells were cultured in the presence of murine M-CSF (10 ng/ml) for 7 days, and then cultured in presence with IFNg 10 ng/ml to induce polarization to M1-like macrophage while M2 like macrophage were induced by IL-4 20 ng/ml for 24 h. YIV-906 or YIV-906GU was added at the same time with IFNg or IL4. The mRNA expression of M1 or M2 related genes were determined by RT-qPCR. Protein expression or phosphorylation were detected with western blotting. Histone H3 was used for normalization of protein loading. Cropped blots are used in this figure and they have been run under the same experimental conditions. Details of experimental procedures are given in “Materials and methods”.