Figure 3
Fenbendazole-induced differentiation of HL60 cells to granulocytes followed by cell death: (a) Cell morphology at 1, 3, 5 and 7 days post treatment with 0.1 µM, 0.2 µM and 0.5 µM of fenbendazole via staining with Wright–Giemsa; (b) necrotic cells; (c) granulocytes; (d) apoptotic cells. (e) Different concentrations of fenbendazole were employed in the NBT reduction assay at different time points. The assay demonstrates that 0.5 µM of fenbendazole increased NBT reduction upon neutrophil differentiation significantly compared to positive control treatment with DMSO). Hence it can be concluded that the mode-of-action hypothesis fenbendazole inducing cell death via differentiation to granulocytes is supported by experiment.
