Figure 7
Pathway analysis of HL-60 cells treated with DMSO and fenbendazole determined by Western Blotting. (a) Expression and phosphorylation levels of AKT, ERK, and STAT3; the quantitative analysis of relative protein level of (b) Total AKT, (c) p-AKT, (d) Total ERK, (e) p-ERK (f) Total STAT3 vs GAPDH; and the relative RNA expression level of (g) AKT, (h) ERK, (i) STAT3, (j) GCSF, and (k) GCSFR following fenbendazole and DMSO (control) treatment, compared to HL-60 cells prior treatment (day 0). *P < 0.05 and **P < 0.01. Each value is presented as mean ± SEM of three independent experiments. As the blots were cropped, full length blots are provided in Supplementary Figure S1.
