Figure 1

Characterization of the norepinephrine (NE) response on JumpIn-NET cells in the TRACT assay. Representative vehicle-corrected normalized Cell Index traces of vehicle-pretreated JumpIn-NET cells (a) in the absence (−dox) or (b) in the presence of 1 µg/ml dox (+dox). (c) Dox-treated cells were pretreated for 1 h with 1 µM nisoxetine prior to stimulation with NE. (d) Combined concentration-effect curves of NE on vehicle- or dox-treated JumpIn-NET cells. Cellular response is expressed as the net AUC of the first 30 min after stimulation. Data are normalized to the response of 100 µM NE on vehicle-treated (–dox) cells. Data are shown as the mean ± SEM of three to eight individual experiments each performed in duplicate.