Figure 4
Assessment of GPCR involvement in substrate-specific cellular responses on dox-treated JumpIn-NET cells. Prior to stimulation with substrate, dox-treated cells were pretreated for 1 h with vehicle or 1 µM antagonist for α2ARs (yohimbine; purple) or βARs (propranolol; mint green) or a 1:1 mixture of both antagonists (light blue). Subsequently, cells were stimulated with an EC80 of either (a,d) norepinephrine (NE, 10 µM; circle), (b,e) dopamine (DA, 100 µM; square), or (c,f) epinephrine (EP, 10 µM; triangle). (a–c) Representative vehicle-corrected normalized Cell Index traces of substrate-induced cellular responses. (d–f) Net AUC of the first 30 min after stimulation. Data are normalized to the response of the respective substrate (100%) in vehicle-pretreated cells. Data are shown as mean ± SEM of three to six individual experiments each performed in duplicate. Comparison of multiple mean values to vehicle control was done using a one-way ANOVA with Dunnett’s post-hoc test. *** p < 0.001 compared to substrate only.
