Figure 4

The distribution of engrafted human γ9δ2 T cells in immunodeficient mice. Adult NOG mice (19–20 weeks old, male) were injected with CFSE-labeled human γ9δ2 T cells (derived from one donor; 1.5 × 10⁷ cells/mouse) via the peritoneal cavity (i.p.; n = 2) or lateral tail vein (i.v.; n = 2), or left un-injected (naive; n = 2). At 24 (a, b) and 48 (c, d) hours post-injection, animals were sacrificed for the isolation of lymphocytes from the heart blood, femur bone marrow (BM), kidney, liver, lung, and spleen. For each group, one animal was sacrificed at each time point. The isolated cells were stained with propidium iodide (PI), and the percentage of viable γ9δ2 T cells (CFSE⁺PI⁻) was determined by the flow cytometry analysis. Data were presented as dot plots with PI versus CFSE fluorescence (a, c), and the percentage of CFSE⁺PI⁻ cells in each sample was plotted in bar charts (b, d). Black bar (■): naive; White bar (□): i.p. injection; Gray bar (■): i.v. injection. Images were created using FlowJo software 10.0.0r1.