Figure 6

(A–I) Immuno-fluorescence detection of γH2AX phosphorylated histone foci in pre-B cells exposed to EMF or X ray irradiation. The γ H2AX foci are shown in bright green—γ-H2AX, whereas DNA staining is in blue – nuclei. (A) Control solvent (DMSO) treated cells Nonirradiated (NIR); (B) Control (DMSO) treated 1 Gy X-ray irradiated cells(X-Ray); (C) Control (DMSO)treated EMF exposed (waves at 950 MHz, with emission generator power setting set at 7 dBm-EMF); (D) IMA treated NIR; (E) IMA, X-ray; (F) IMA, EMF; (G) GSK, NIR; (H) GSK, X-ray; (I) GSK, EMF. (J) Number of counted foci per /cell represented as histograms. W1/4 refers to growing plate wells 1 and 4, W2/5 wells 2 and 5 and W3/6 wells 3 and 6 equivalent positions with respect to which cells were EMF irradiated, with generator power setting set at 7 dBm and 13 dBm. GSK is cells treatment with 10 µM GSK-690693, IMA their treatment with 3 µM Imatinib. ANDOR camera assisted by IQ Live Cell Imaging software and foci analysis with Imaris for Cell Biologists software (both from OXFORD Instruments).