Figure 3

BLI of ACE2(WT) or Triple Decoy for mutated RBD and inhibition in the neutralization assay. (a–r) BLI of WT or Triple Decoy ACE2 to WT or mutated S RBD are shown side-by-side. (s) The percent inhibition of RBD binding to ACE2 in the surrogate neutralization assay is shown for the ACE2 Triple Decoy with S RBD WT and listed variants. RBD concentrations were 25 μg/mL. The negative control is no decoy. Inhibition of ≥ 30% (dashed line) correlates with neutralization of the virus. Data graphed as mean with SEM. Statistics: one-way ANOVA and Tukey’s post-hoc analysis comparing Triple Decoy binding to RBD WT/variants. For RBD K417N vs WT, p = 0.0495; vs L452R, p = 0.0451; and vs E484K/K417N/N501Y, p = 0.0128.