Figure 1
From: Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
Schematic display of SELEX steps. (A) Schematic display of the preparation CCHFV NP-MB. The C-terminal of viral recombinant NP was biotinylated by the BirA enzyme and was fixed onto the surface of the streptavidin-coated magnetic beads. (B) ssDNA aptamers were mixed with Target; probable combinations of magnetic particles-NP-Aptamers (MB-NP-Apt) were deposited with a magnet. The attached aptamers were separated by heat giving after the sedimentation. The ssDNA bounded aptamers were amplified by PCR and were single-stranded by lambda exonuclease enzyme and entered the next round of SELEX. The PCR product of the optimum round was cloned and sequenced.
