Figure 2

Study timeline detailing the experimental protocol. Gene expression, proliferation and migration of tenocytes of all five species (human, mouse, rat, sheep, horse) were compared under standard culture conditions (healthy control) as well as under transient (24 h) and constant exposure to inflammatory stimuli (10 ng/ml IL1β and 10 ng/ml TNFα). After 24-h exposure to inflammation, the transient inflammation group received fresh culture medium, while for the constant inflammation group fresh medium was again supplemented with inflammatory factors.