Figure 5
From: Venom duct origins of prey capture and defensive conotoxins in piscivorous Conus striatus
Visualisation of the venom distribution pattern across eight venom duct sections and their correlation to the injected predatory and defensive venoms obtained from the same specimen of C. striatus (specimen D). (a) MALDI spot imaging showing the alignment of the injected predatory and defensive venoms with extracted venoms of eight venom duct sections of the injected and extracted venoms. (b) Quantitative analysis of the major peptides found in the LC/MS profiles of the dissected venoms (venoms extracted from 8 duct segments). Both the retention time and the mass was considered when identifying the peptides across the duct sections and injected venoms. Peptides are ranked to the expression levels (relative to the maximum intensity of each sample) of section 1 (left) and 7 (right). (c) Mass profile comparisons were made between the LC/MS data obtained by injecting similar amount (1 μl) of the injected predatory and defensive venoms in comparison the distal duct section 2 and proximal duct section 7. Peptides are ranked by expression level (relative to the maximum intensity of each sample) of section 2 (left) and 7 (right). The inset Venn diagram shows the unique and the shared masses in each venom. Reconstructed LC/MS chromatograms were used to analyse the mass profiles and their expression levels. Figure 5b,c are also shown in heat map versions in supplementary Figure S6.
