Table 1 Integration of the transcriptome and proteomic data using ProteinPilot tool. Underlined sequences are matched to 99% confidence (list of peptide fragments are shown in supplementary table S8) to the proteomic data obtained from the three venom duct sections of C. striatus. MS/MS fragments obtained from reduced-alkylated and reduced-alkylated-trypsin digested extracted venom from three venom duct sections of specimens A, B and C of C. striatus were used to map their presence in the proteome.
From: Venom duct origins of prey capture and defensive conotoxins in piscivorous Conus striatus
Sequence ID | Superfamily | Mature sequence* | Distal | Central | Proximal |
|---|---|---|---|---|---|
STR1_SI | A | ICCNPACGPKYSC | ✓ | ✓ | ✓ |
STR14_Sm1.2 | A | NGCCRNPACESHRC | ✓ | ✓ | |
STR17_SII | A | GCCCNPACGPNYGCGTSCS | ✓ | ✓ | ✓ |
STR18 | A | APALVVTATTNCCGYTGPACHPCLCTQTC | ✓ | ||
STR19 | A | QKELVPSVITTCCGYDPGTMCPPCRCDNSCKPKPKK | ✓ | ||
STR20_SIVB | A | QKELVPSVITTCCGYDPGTMCPPCRCTNSC | ✓ | ||
STR21_SIVA | A | QKSLVPSVITTCCGYDPGTMCPPCRCTNSC | ✓ | ✓ | |
STR22 | A | QKSLVPSVITTCCGYDPGTMCPPCRCTNSCPKKPKKP | ✓ | ||
STR24_Sx4.1 | A | QKSLVPSVITTCCGYDPGTMCPPCRCTNSC | ✓ | ||
STR25 | A | QKSLVPSVITTCCGYDPGTMCPPCRCTNSCKTKPKK | ✓ | ||
STR26 | A | QKELVPSVITTCCGYDPGTMCPPCRCTNSCKTKPKK | ✓ | ||
STR34 | B2 | KQHSQFNADENKAAFDSEDSLGNFMDFLHNEKGDKLPFANVDSAATDLGQFQPSAENEDGKFRFFDRQQ | ✓ | ✓ | ✓ |
STR36 | B2 | KQHSQFNADENKAAFDSEDSLGNFMDFLHNEKGDKVPFANVDSAATDLGQFQPSAENEDGKFRFFDRQQ | ✓ | ✓ | ✓ |
STR37 | B2 | KQHSQFNADENKAAFDSEDSLGNFMDFLHNEKGDKVPFANVDSAATDLGQFQPSAENEDGKFRFFDRQQ | ✓ | ||
STR39 | B2 | KQHSQFNADENKAAFDSEDSLGNFMDFLHNEKGDKLPFANVDSAAADLGQFQPSAENEDGKFRFFDRQQ | ✓ | ||
STR41 | Con-ikot-ikot | SGPADCCRMKECCTDRVNECLQRYSGREDKFVSTCYQEATLTCGSFNEIVGCCYGYQMCMIRVVKPNSLSGAHEACKTVSCGNPCA | ✓ | ||
STR42_Con-ikot-ikot_SI | Con-ikot-ikot | SGPADCCRMKECCTDRVNECLQRYSGREDKFVSFCYQEATVTCGSFNEIVGCCYGYQMCMIRVVKPNSLSGAHEACKTVSCGNPCA | ✓ | ✓ | ✓ |
STR52 | Conkunitzin | DRPSLCDLPADSGSGTKAEKRIYYNSARKQCLRFDYTGQGGNENNFRRTYDCQRTCLYT | ✓ | ||
STR53 | Conkunitzin | PSYCNLPADSGSGTKPEQRIYYNSAKKQCVTFTYNGKGGNGNNFSRTNDCRQTCQYPA | ✓ | ||
STR54 | Conkunitzin | PSYCNLPADSGSGTKPEQRIYYNSAKKQCVTFTYNGKGGNGNNFSRTNDCRQTCQYPLYACISGCRCET | ✓ | ||
STR55_Conkunitzin-S2 | Conkunitzin | ARPKDRPSYCNLPADSGSGTKPEQRIYYNSAKKQCVTFTYNGKGGNGNNFSRTNDCRQTCQYPV | ✓ | ✓ | |
STR56_Conkunitzin-S1 | Conkunitzin | KDRPSLCDLPADSGSGTKAEKRIYYNSARKQCLRFDYTGQGGNENNFRRTYDCQRTCLYT | ✓ | ✓ | |
STR66 | conopressin | CIIRNCPRGGKRDVDETHLTMPCMCCSFRQCGAEYLLWSWRMGNGDRRSDQVH | ✓ | ✓ | |
STR67 | Conophysin | CIIRNCPRGGKRDVDETHLTMPCMCCSFRQCGAPYLLWSWRMGNGDRRSDQVH | ✓ | ||
STR70 | G2 | DCQRGCVGCGNRAGCCCGNKYCDKDNTCQEKPAKPST | ✓ | ||
STR73 | H | DSPQSECDGPRCPFICCFYEERKCGTRDCP | ✓ | ||
STR76 | I1 | GTCSGVEQQCSNNADCCGELCCLSDKCGSPCMIRL | ✓ | ✓ | ✓ |
STR105 | M | CCIAPMCRGPCKCCEEPGHP | ✓ | ||
STR107_S3-S02 | M | CCPARMCMAACSCCD | ✓ | ||
STR115_S3-G04 | M | QKCCGEGSSCPKYFKNNFICGCC | ✓ | ✓ | |
STR116_SIIIB | M | QNCCNGGCSSKWCKGHARCC | ✓ | ✓ | |
STR117_SIIIA | M | QNCCNGGCSSKWCRDHARCC | ✓ | ✓ | |
STR144_Conotoxin-3 | O1 | CESYGKPCGIYNDCCNACDPAKKTCT | ✓ | ✓ | ✓ |
STR146_SO3 | O1 | CKAAGKPCSRIAYNCCTGSCRSGKC | ✓ | ✓ | |
STR147_S6.1 | O1 | CKAAGKSCSRIAYNCCTGSCRSGKC | ✓ | ✓ | |
STR148_SVIB | O1 | CKLKGQSCRKTSYDCCSGSCGRSGKC | ✓ | ✓ | |
STR150 | O1 | CRPSGSNCGNISICCGRCVNRRCT | ✓ | ✓ | |
STR151_SVIA mutant 1 | O1 | CRPSGSPCGVTSICCGRCYRGKCT | ✓ | ✓ | |
STR152_SVIA | O1 | CRSSGSPCGVTSICCGRCYRGKCT | ✓ | ✓ | ✓ |
STR157 | O1 | DCGEGGQGCYTRPCCPGRECVAGATGGGVCL | ✓ | ✓ | |
STR183_S6.8 | O1 | DGCSNAGGFCGIHPGLCCSEICLVWCT | ✓ | ||
STR184_SVIE | O1 | DGCSSGGTFCGIHPGLCCSEFCFLWCITFID | ✓ | ||
STR186 | O1 | DKQEYHAVRKWSCVKRGDSCKTNICCAGLTCLRAHAINICLYLMPI | ✓ | ||
STR187_Mr022 | O1 | ECREKGQGCTNTALCCPGLECEGQSQGGLCVDN | ✓ | ||
STR207 | O1 | KSWSCVEHGDSCKTNICCAGLTCLRAHAINLCLYLMPM | ✓ | ✓ | |
STR216-SO4 | O1 | STTKVSKATDCIEAGNYCGPTVMKICCGFCSPYSKICMNYPKN | ✓ | ✓ | |
STR218_SO5 | O1 | STTKVSKSTSCMEAGSYCGSTTRICCGYCAYFGKKCIDYPSN | ✓ | ✓ | |
STR221 | O1 | VRESDSCRKLGERCPSRPCCPRLRCGSGRAGGVCRHPYN | ✓ | ✓ | |
STR222 | O1 | VRESEGCAGLGAPCRYRRCCRRLKCVGGHVGRACRYPANYYYYY | ✓ | ✓ | |
STR229_contryphan-G | contryphan | GCPWEPWC | ✓ | ✓ | |
STR249 | O2 | KNCAYAFDACTSDRQCCSGYCVGNVYCE | ✓ | ✓ | ✓ |
STR252 | O2 | KSNAESWWEGECRTWNAPCSFTSQCCFGKCAHHRCIAW | ✓ | ✓ | ✓ |
STR259 | O2 | IYYNSARKQCLRFDYTGQGGNENNFRRTYDCQRTCLYT | ✓ | ✓ | ✓ |
STR297 | O3 | DKQEYHAVRKWSCVKRGDSCKTNICCAGLTCLRARAINVCLYLMPI | ✓ | ||
STR301 | O3 | NTPDDGTCKSSSNCSTGQTCCKANAKNEKGFCTEDCWF | ✓ | ||
STR303 | O3 | TADEACKEYCEERNKNCCGRTNGEPRCASMCF | ✓ | ✓ | |
STR312 | O3 | TVDEECKEYCEQRNKNCCGETNGEPVCAQACL | ✓ | ✓ | |
STR320 | P | RVNCAGTLCQNGKCGGDCICRPANSTHQDCQPNDFD | ✓ | ✓ | |
STR321 | P/O2 | KSNAESWWEGECRTWNAPCSSTSQCCFGRCAHHRCIAW | ✓ | ||
STR326 | Sf-Mi2 | DCQRGCVGCGNRAGCCCGNKYCDKDNTCQPNPVWL | ✓ | ✓ | ✓ |
STR333 | T | HCCPIDLPCCPL | ✓ | ||
STR337 | W | ISKSMGDVVGRTWWCPPEGELTHAGSATKQLLSSVWGLIGGVLRMLDQNRRH | ✓ | ✓ | ✓ |
