Figure 2 | Scientific Reports

Figure 2

From: GMP compliant isolation of mucosal epithelial cells and fibroblasts from biopsy samples for clinical tissue engineering

Figure 2

Epithelial cells digestion. The Porcine epithelial cell sheets were digested (a) either manually or automatically and the cell number counted (a). Data shows mean ± SD, n = 4. Comparison of epithelial cells automatically digested, grown on primary feeder layers in FBS, HPL or HPL Y-27632 medium. Cell number (b) viability (c) and population doublings (d) were recorded up to passage 3. Data shows mean ± SD; n = 3. *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001 except d, which shows median + interquartile range. Cells grown in media containing FBS (e), HPL (f) and HPL plus Y-27632 (g). n = 4, scale bar shows 200 µm.

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