Figure 3 | Scientific Reports

Figure 3

From: GMP compliant isolation of mucosal epithelial cells and fibroblasts from biopsy samples for clinical tissue engineering

Figure 3

Comparison growth conditions. Porcine epithelial cells grown in the 4 conditions 3T3 FBS Y-27632, 3T3 HPL Y-27632, Primary FBS Y-27632, Primary HPL Y-27632 were compared. Viability (a), at passage 2 3T3 FBS is significantly reduced compared to those grown on primary feeder layers, cumulative cell number (b), population doubling time, data shows median plus interquartile range (c) and cumulative population doublings. Graphs show mean plus SD *P < 0.05, n = minimum of 3. xCELLigence growth at each passage 1 (d) passage 2 (e) Passage 3 (f). Wells were baselined to those containing feeder cells. Cell index is a measure of electrical impedance. Graphs show mean, Green shows 3T3 FBS, Blue shows 3T3 HPL, Black shows Primary FBS and Red shows Primary HPL. There is no significant difference between conditions at 160 h. n   =  3.

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