Figure 4 | Scientific Reports

Figure 4

From: GMP compliant isolation of mucosal epithelial cells and fibroblasts from biopsy samples for clinical tissue engineering

Figure 4

Stem cell populations. Stem cell analysis was carried out on porcine epithelial cells grown in the 4 conditions 3T3 FBS Y-27632, 3T3 HPL Y-27632, Primary FBS Y-27632, Primary HPL Y-27632,for Colony formation efficiency (CFE) assay all colonies were counted, by passage 3 those on primary HPL Y-27632 are significantly reduced compared to FBS 3T3 Y-27632 *P > 0.05 (a). Holoclones colonies larger than 10 mm with round edges were counted, there was no significant difference between the conditions (b). P75 was measure using Flow cytometry at passage 3. There was no significant difference between the conditions (c). Data shows mean plus SD, n = 3.

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