Table 4 Summary of molecular analysis of randomly selected transgenic sugarcane plants (V1-generation).
From: Novel approaches to circumvent the devastating effects of pests on sugarcane
Transgenic lines of sugarcane (CPF-246) | PCR | Southern Blot | Dipstick assay | ELISA (µg/g) | Glyphosate spray assay (1200 mL/acre) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTG | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTG | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTG | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTG | ||
CPF-246 (2L/6-3) 1 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.490 | 0.411 | 0.451 | Tolerant |
CPF-246 (2L/8-5) 2 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.410 | 0.480 | 0.432 | Tolerant |
CPF-246 (3L/3-8) 3 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.510 | 0.441 | 0.421 | Tolerant |
CPF-246 (3L/4-4) 4 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.464 | 0.542 | 0.434 | Tolerant |
CPF-246 (4L/2-7) 5 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.672 | 0.642 | 0.569 | Tolerant |
CPF-246 (4L/7-1) 6 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.456 | 0.467 | 0.459 | Tolerant |
CPF-246 (4L/8-4) 7 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.552 | 0.560 | 0.454 | Tolerant |
CPF-246 (5L/1-7) 8 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.345 | 0.423 | 0.458 | Tolerant |
CPF-246 (5L/5-2) 9 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.678 | 0.599 | 0.578 | Tolerant |
CPF-246 (6L/2-10) 10 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.458 | 0.523 | 0.455 | Tolerant |
CPF-246 (6L/5-3) 11 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.687 | 0.611 | 0.589 | Tolerant |
CPF-246 (7L/2-1) 12 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.489 | 0.478 | 0.458 | Tolerant |
CPF-246 (7L/7-7) 13 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.521 | 0.455 | 0.404 | Tolerant |
CPF-246 (L8/4-10) 14 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.642 | 0.648 | 0.551 | Tolerant |
CPF-246 (L9/6-9) 15 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.652 | 0.622 | 0.564 | Tolerant |
