Table 5 Molecular analysis of CPF-246 (L4/2-9) transgenic sugarcane plants from V2-generation.
From: Novel approaches to circumvent the devastating effects of pests on sugarcane
Serial no | PCR | Southern Blot analysis | Dipstick assay | ELISA (µg/g) | Glyphosate spray assay (1200 m/acre) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Transgenic lines of sugarcane CPF-246 | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | |
CPF-246 (4L/2-9) 1 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.675 | 0.645 | 0.565 | Tolerant |
CPF-246 (4L/2-9) 2 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.677 | 0.641 | 0.561 | Tolerant |
CPF-246 (4L/2-9) 3 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.598 | 0.596 | 0.551 | Tolerant |
CPF-246 (4L/2-9) 4 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.645 | 0.644 | 0.560 | Tolerant |
CPF-246 (4L/2-9) 5 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.678 | 0.656 | 0.562 | Tolerant |
CPF-246 (4L/2-9) 6 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.670 | 0.651 | 0.567 | Tolerant |
CPF-246 (4L/2-9) 7 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.671 | 0.655 | 0.570 | Tolerant |
CPF-246 (4L/2-9) 8 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.612 | 0.634 | 0.559 | Tolerant |
CPF-246 (4L/2-9) 9 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.615 | 0.629 | 0.545 | Tolerant |
CPF-246 (4L/2-9) 10 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.679 | 0.642 | 0.568 | Tolerant |
