Table 6 Molecular analysis of CPF-246 (L5/5-8) transgenic sugarcane plants from V2-generation.
From: Novel approaches to circumvent the devastating effects of pests on sugarcane
Serial no | PCR | Southern Blot analysis | Dipstick asaay | ELISA (µg/g) | Glyphosate spray assay (1200 m/acre) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Transgenic lines of sugarcane CPF-246 | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | |
CPF-246 (5L/5-8) 1 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.680 | 0.598 | 0.577 | Tolerant |
CPF-246 (5L/5-8) 2 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.670 | 0.590 | 0.560 | Tolerant |
CPF-246 (5L/5-8) 3 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.676 | 0.595 | 0.575 | Tolerant |
CPF-246 (5L/5-8) 4 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.678 | 0.601 | 0.572 | Tolerant |
CPF-246 (5L/5-8) 5 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.674 | 0.596 | 0.580 | Tolerant |
CPF-246 (5L/5-8) 6 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.670 | 0.590 | 0.578 | Tolerant |
CPF-246 (5L/5-8) 7 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.665 | 0.580 | 0.581 | Tolerant |
CPF-246 (5L/5-8) 8 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.673 | 0.598 | 0.572 | Tolerant |
CPF-246 (5L/5-8) 9 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.675 | 0.591 | 0.570 | Tolerant |
CPF-246 (5L/5-8) 10 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.678 | 0.588 | 0.575 | Tolerant |
