Table 8 Molecular analysis of CPF-246 (L8/4-3) transgenic sugarcane plants from V2-generation.
From: Novel approaches to circumvent the devastating effects of pests on sugarcane
Serial no | PCR | Southern Blot analysis | Dipstick assay | ELISA (µg/g) | Glyphosate spray assay (1200 ml/acre) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Transgenic lines of sugarcane CPF-246 | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | |
CPF-246 (L8/4-3) 1 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.645 | 0.640 | 0.551 | Tolerant |
CPF-246 (L8/4-3) 2 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.640 | 0.644 | 0.542 | Tolerant |
CPF-246 (L8/4-3) 3 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.641 | 0.642 | 0.534 | Tolerant |
CPF-246 (L8/4-3) 4 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.640 | 0.622 | 0.538 | Tolerant |
CPF-246 (L8/4-3) 5 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.630 | 0.635 | 0.525 | Tolerant |
CPF-246 (L8/4-3) 6 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.638 | 0.635 | 0.535 | Tolerant |
CPF-246 (L8/4-3) 7 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.642 | 0.632 | 0.552 | Tolerant |
CPF-246 (L8/4-3) 8 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.635 | 0.638 | 0.551 | Tolerant |
CPF-246 (L8/4-3) 9 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.635 | 0.640 | 0.540 | Tolerant |
CPF-246 (L8/4) 10 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.641 | 0.646 | 0.550 | Tolerant |
