Table 9 Molecular analysis of CPF-246 (L9/6-10) transgenic sugarcane plants from V2-generation.
From: Novel approaches to circumvent the devastating effects of pests on sugarcane
Serial no | PCR | SOUTHERN | DIPSTICK | ELISA (µg/g) | Glyphosate spray assay (1200 m/acre) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Transgenic lines of sugarcane CPF-246 | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | |
CPF-246 (L9/6-10) 1 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.648 | 0.620 | 0.565 | Tolerant |
CPF-246 (L9/6-10) 2 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.646 | 0.611 | 0.561 | Tolerant |
CPF-246 (L9/6-10) 3 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.644 | 0.603 | 0.558 | Tolerant |
CPF-246 (L9/6-10) 4 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.650 | 0.599 | 0.555 | Tolerant |
CPF-246 (L9/6-10) 5 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.640 | 0.598 | 0.560 | Tolerant |
CPF-246 (L9/6-10) 6 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.645 | 0.599 | 0.559 | Tolerant |
CPF-246 (L9/6-10) 7 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.641 | 0.610 | 0.556 | Tolerant |
CPF-246 (L9/6-10) 8 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.639 | 0.596 | 0.567 | Tolerant |
CPF-246 (L9/6-10) 9 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.633 | 0.613 | 0.560 | Tolerant |
CPF-246 (L9/6-10) 10 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.640 | 0.615 | 0.562 | Tolerant |
