Table 1 Segregation pattern of crtRB1 3ˊTE, lcyE 5ˊTE and o2 alleles in different backcross and self- generations of the four crosses.

From: Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids

Cross

Generation

N

C + C + 

C + C

CC

χ2

P-value

N

L + L + 

L + L

LL

χ2

P-value

N

O + O + 

OO

χ2

P-value

QLM11 × HP467-15

BC1F1

390

210

180

 

2.31

0.13 ns

180

105

75

 

5.00

0.03*

75

40

35

0.33

0.56 ns

BC2F1

188

115

73

 

9.38

0.0022*

73

45

28

 

3.96

0.05*

28

 

28

  

BC2F2

655

231

298

126

38.98

 0.00001*

126

35

70

21

4.67

0.10 ns

21

 

21

  

QLM12 × HP467-15

BC1F1

434

236

197

 

3.51

0.06 ns

197

102

95

 

0.25

0.62 ns

95

50

45

0.26

0.61 ns

BC2F1

204

134

70

 

20.08

0.00001*

70

38

32

 

0.51

0.47 ns

32

 

32

  

BC2F2

725

245

325

155

30.10

0.00001*

155

43

82

30

2.70

0.26 ns

30

 

30

  

QLM13 × HP467-15

BC1F1

486

276

210

 

8.96

0.0027*

210

100

110

 

0.48

0.49 ns

110

61

49

1.31

0 .25 ns

BC2F1

364

235

129

 

30.87

0.00001*

129

77

52

 

4.85

0.03*

52

 

52

  

BC2F2

441

132

194

115

7.68

0.0215*

115

29

61

25

0.70

0.70 ns

25

 

25

  

QLM14 × HP467-15

BC1F1

270

177

93

 

26.13

0.00001*

93

49

44

 

0.27

0.60 ns

44

25

19

0.81

0 .36 ns

BC2F1

265

170

95

 

21.23

0.00001*

95

52

43

 

0.85

0.36 ns

43

 

43

  

BC2F2

312

84

157

71

1.10

0.58 ns

71

20

34

17

0.38

0.83 ns

17

 

17

  
  1. *Significant at P = 0.05; ns, non-significant; N, No. of plants genotyped; df, degrees of freedom; C + , unfavorable allele of crtRB1; C, favorable allele of crtRB1; L + , unfavorable allele of lcyE; L, favorable allele of lcyE; O + , unfavorable allele of o2; O, favorable allele of o2.