Figure 5
Immunofluorescence or DCFA staining of HepaRG cells cultured on the 40–2 µm device, revealed under epifluorescent or confocal microscopy. Top-down views of the chamber with respect to proliferative HepaRG cells loaded and cultured for 14 days (a) fixed and stained for nuclei (blue), F-actin (green), HNF4α (red) and albumin (grey) or (b) at 50 nl/min, stained for DCFA (green) and examined under phase contrast imaging. (c) HepaRG hepatocytes loaded and cultured for 21 days fixed and stained for nuclei (blue), F-actin (green), ZO-1 (red), and albumin (grey). Some binucleated cells can also be seen (white arrows). Scale bars = 20 µm.
