Figure 1 | Scientific Reports

Figure 1

From: Susceptibility of cyclin-dependent kinase inhibitor 1-deficient mice to rheumatoid arthritis arising from interleukin-1β-induced inflammation

Figure 1

p21-deficient mice exhibit destructed knee joint, increased inflammatory arthritis, and enhanced systemic inflammatory cytokine expression in vivo. (a) OARSI-based arthritis scores of p21−/− and p21+/+ mice on days 7, 10, 14, and 28 after administration of the antibody cocktail. Cartilage and synovial tissue samples were collected from the knee and stained with (b) Safranin-O and Fast Green, and (d) hematoxylin and eosin, respectively. (b,d) p21+/+ mice and p21−/− mice as controls and on days 7, 14, and 28. (c) Average sum of the OARSI cartilage OA-histopathology scores and (e) average severity of synovitis scores with 95% CI from four quadrants (i.e., medial femoral condyle, medial tibial plateau, lateral femoral condyle, and lateral tibial plateau) and eight sections per knee. Four mice were analyzed from each group. (f) Serum levels of IL-1β, IL-6 and TNF-α of p21−/− and p21+/+ mice at each time point and control. In (c,e,f), four mice were analyzed from each group. Two-way analysis of variance and Tukey’s post hoc test for multiple comparisons of paired samples were used. CI confidence interval, CAIA collagen antibody-induced arthritis, p21 cyclin-dependent kinase inhibitor 1.

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