Figure 3
Schematic picture of telomerase RNA and template regions in Saccharomyces cerevisiae, Zygosaccharomyces bailii, Z. rouxii, and Z. sapae. We identified the same putative consensus telomere sequence as 5′-ACACCC (or its permutations) in Saccharomyces cerevisiae, Zygosaccharomyces bailii and Z. rouxii, although the template regions, annealing and telomere synthesis during reverse transcription differed in all three species. The corresponding template regions are indicated in bold in the sequences (direct repeats), and underlined fonts (template sequence), respectively. (1) In S. cerevisiae the maximum template region consists of two adjacent CCACAC repeats. (2) In Z. bailii the template region is formed by a shorter sequence than in S. cerevisiae and enables the annealing of five nucleotides and the addition of six nucleotides complementary to ACACCC. (3) The template region in Z. rouxii is a bit paradoxical. The synthesis is probably running on the template sequence CACCCA (a permutated version of ACACCC) and annealing occurs through a single base pair (the first A in the template). However, the template region from Z. rouxii corresponds exactly to a much longer telomere sequence (21 nt) from the species Z. sapae, with a potentially longer annealing (up to 8 bp). Three TR paralogs were found in Z. sapae. (Figure created using Adobe Photoshop 22.2.0).
