Figure 4
Thioflavin T (ThT) fluorescence assay. We tested four oligonucleotides representing selected telomere sequences from Sachcaromyces sp., Zygosaccharomyces sp., Lachancea sp., Torulaspora sp., and Kluyveromyces marxianus; three controls—one G4-forming sequence (called 4GC) and two negative controls (polyA and random). The G4 propensity was confirmed in vitro by both methods in three out of four telomere sequences tested as well as in positive control (in this figure, Supplementary table S4). The only sample sequence which did not form G4 in vitro was the one that was negative by prediction also. (Figure created using Adobe Photoshop 22.2.0).
