Figure 5
Supplementation with SCN− increases content of collagen. Panels (A) and (B) show representative Trichrome-stained images taken at the site of ligation at 24 h post surgery from: (A) a vehicle/IR rat, and (B) a SCN–supplemented/IR rat, with the blue staining depicting collagen. (C) shows the quantification of collagen areas from 6 sections taken between the ligation site and apex in each heart from the control (black bar, n = 7 rats) and SCN–supplemented (grey bar, n = 6 rats) groups with * showing a significant (p < 0.05) difference between groups using a one-tailed t-test. (D) and (E) Quantification of collagen area was performed on individual sections taken from the site of ligation to the apex for control (black line) and SCN− (grey line) groups with data in (E) expressed as percentage of the total stained area in the section. Data represent mean ± SEM of n ≥ 6 rats with * showing a significant difference between the vehicle/IR animals and the SCN− -supplemented/IR group, using 2-way ANOVA with a Sidak post-hoc test.
